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        If you use plots from MultiQC in a publication or presentation, please cite:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411
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        Tool Citations

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        About MultiQC

        This report was generated using MultiQC, version 1.31

        You can see a YouTube video describing how to use MultiQC reports here: https://youtu.be/qPbIlO_KWN0

        For more information about MultiQC, including other videos and extensive documentation, please visit http://multiqc.info

        You can report bugs, suggest improvements and find the source code for MultiQC on GitHub: https://github.com/MultiQC/MultiQC

        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

        Report generated on 2025-11-12, 12:18 CST based on data in:
        • /data/users/willetse0745/Planaria-Genome-Project/fastq_planaria
        • /data/users/willetse0745/Planaria-Genome-Project/trimmed_fastq/qc
        • /data/groups/wheelenj/sequencing/20250916_M009242/busco_test/short_summary.specific.metazoa_odb10.busco_test.txt

        General Statistics

        Showing 3/3 rows and 3/12 columns.
        Sample NameComplete BUSCOsComplete and single-copy BUSCOsComplete and duplicated BUSCOsFragmented BUSCOsMissing BUSCOsMedian lengthMean lengthRead N50Median QualMean Qual# Reads (K)Total Bases (Mb)
        busco_test | short_summary.specific.metazoa_odb10.busco_test
        270.0
        245.0
        25.0
        76.0
        608.0
        qc | NanoStats
        9585bp
        13166bp
        16392bp
        20.7
        18.4
        372.0K
        4898.0Mb
        qc | NanoStats_raw
        3033bp
        6122bp
        13039bp
        18.6
        16.7
        1000.0K
        6122.5Mb

        BUSCO

        Version: 6.0.0

        Assesses genome assembly and annotation completeness.URL: http://busco.ezlab.orgDOI: 10.1093/bioinformatics/btv351

        BUSCO v2 provides quantitative measures for the assessment of genome assembly, gene set, and transcriptome completeness, based on evolutionarily-informed expectations of gene content from near-universal single-copy orthologs selected from OrthoDB v9.

        Lineage: metazoa_odb10

        Created with MultiQC

        NanoStat

        Reports various statistics for long read dataset in FASTQ, BAM, or albacore sequencing summary format (supports NanoPack; NanoPlot, NanoComp).URL: https://github.com/wdecoster/nanostat; https://github.com/wdecoster/nanoplotDOI: 10.1093/bioinformatics/bty149

        Programs are part of the NanoPack family for summarising results of sequencing on Oxford Nanopore methods (MinION, PromethION etc.)

        Summary Statistics (FASTQ)

        Showing 2/2 rows and 5/7 columns.
        Sample NameMedian lengthMean lengthRead N50Median QualMean Qual# Reads (K)Total Bases (Mb)
        qc | NanoStats
        9585bp
        13166bp
        16392bp
        20.7
        18.4
        372.0K
        4898.0Mb
        qc | NanoStats_raw
        3033bp
        6122bp
        13039bp
        18.6
        16.7
        1000.0K
        6122.5Mb

        Reads by quality

        Read counts categorised by read quality (Phred score).

        Sequencing machines assign each generated read a quality score using the Phred scale. The phred score represents the liklelyhood that a given read contains errors. High quality reads have a high score.

        Created with MultiQC

        Software Versions

        Software Versions lists versions of software tools extracted from file contents.

        SoftwareVersion
        BUSCO6.0.0

        MultiQC v1.31 - Written by Phil Ewels, available on GitHub.

        This report uses Plotly, jQuery, jQuery UI, Bootstrap and FileSaver.js.